Methylation assay by nucleotide incorporation: a quantitative assay for regional CpG methylation density
نویسندگان
چکیده
منابع مشابه
Methylation assay by nucleotide incorporation: a quantitative assay for regional CpG methylation density.
Although the aberrant methylation in CpG islands is of great interest as a causative role in human malignancies, it has been very difficult to accurately determine methylation density. Here we report a novel microplate-based quantitative methylation assay, designated MANIC, for a region containing a number of CpG sites based on incorporation of hapten-labeled dCTP at cytosine sites where the me...
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Aberrant methylation of promoter CpG islands is causally linked with a number of inherited syndromes and most sporadic cancers, and may provide valuable diagnostic and prognostic biomarkers. In this report, we describe an approach to simultaneous analysis of multiple CpG islands, where methylation-specific oligonucleotide probes are joined by ligation and subsequently amplified by polymerase ch...
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ژورنال
عنوان ژورنال: BioTechniques
سال: 2004
ISSN: 0736-6205,1940-9818
DOI: 10.2144/04365rr02